9B0Z
Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.96863 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 247.573, 72.313, 56.345 |
Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
Resolution | 44.180 - 2.410 |
R-factor | 0.2231 |
Rwork | 0.221 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.813 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.440 | 2.830 |
High resolution limit [Å] | 2.400 | 2.700 |
Rmerge | 0.087 | 0.630 |
Rmeas | 0.106 | 0.769 |
Rpim | 0.059 | 0.435 |
Total number of observations | 10863 | |
Number of reflections | 27383 | 3582 |
<I/σ(I)> | 7.6 | 1.2 |
Completeness [%] | 99.6 | |
Redundancy | 3.1 | 3 |
CC(1/2) | 0.989 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 20% PEG 2K MME, 0.2 M TAO and 0.1 M Tris pH 8.5. Cryoprotected in mother liquor containing 20% glycerol |