9B0B
Structure of Optineurin bound to HOIP NZF1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96859 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.755, 92.684, 149.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.265 - 1.700 |
R-factor | 0.1974 |
Rwork | 0.196 |
R-free | 0.22170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.527 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.820 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.081 | 0.830 |
Rmeas | 0.088 | 0.904 |
Rpim | 0.035 | 0.355 |
Total number of observations | 493784 | 25734 |
Number of reflections | 77197 | 4026 |
<I/σ(I)> | 12.1 | 1.9 |
Completeness [%] | 100.0 | |
Redundancy | 6.4 | 6.4 |
CC(1/2) | 0.999 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 3:1 (protein:mother liquor: 10% PEG 8K, 20% ethylene glycol, 0.1 M Tris/Bicine pH 8.5, 0.03 M MgCl2 and 0.03 M CaCl2. cryoprotected in mother liquor containing 10% glycerol |