9B00

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with berberine analog of chloramphenicol CAM-BER, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.80A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	208.470, 447.660, 619.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	124.260 - 2.800
R-factor	0.232
Rwork	0.229
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.866
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	124.260	124.260	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.200	0.029	1.969
Rmeas	0.222	0.032	2.187
Total number of observations	7190260
Number of reflections	1383303	15863	102443
<I/σ(I)>	8.34	41.63	0.9
Completeness [%]	98.6	95.3	99.1
Redundancy	5.198	5.391	5.199
CC(1/2)	0.994	0.998	0.162

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME