9B00

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with berberine analog of chloramphenicol CAM-BER, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.80A resolution

This is a non-PDB format compatible entry.

Summary for 9B00

• Entry DOI: 10.2210/pdb9b00/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (61 entities in total)
• Functional Keywords: chloramphenicol; berberine; antibiotic; 70s ribosome; x-ray structure; translation inhibitor; binding affinity; peptidyl transferase center; nascent peptide exit tunnel, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4569739.59

Authors

Batool, Z.,Pavlova, J.A.,Paranjpe, M.N.,Tereshchenkov, A.G.,Lukianov, D.A.,Osterman, I.A.,Bogdanov, A.A.,Sumbatyan, N.V.,Polikanov, Y.S. (deposition date: 2024-03-11, release date: 2024-08-07, Last modification date: 2024-09-25)

• Primary citation: Batool, Z.,Pavlova, J.A.,Paranjpe, M.N.,Tereshchenkov, A.G.,Lukianov, D.A.,Osterman, I.A.,Bogdanov, A.A.,Sumbatyan, N.V.,Polikanov, Y.S.; Berberine analog of chloramphenicol exhibits a distinct mode of action and unveils ribosome plasticity.; Structure, 32:1429-, 2024; PubMed: 39019034; DOI: 10.1016/j.str.2024.06.013

Experimental method

X-RAY DIFFRACTION (2.8 Å)