8ZR2

Cocrystallization of engineered streptavidin with C9 oligo DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-08
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	80.937, 80.986, 77.316
Unit cell angles	90.00, 91.97, 90.00

Refinement procedure

Resolution	46.540 - 2.500
R-factor	0.2399
Rwork	0.234
R-free	0.34560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lng
RMSD bond length	0.008
RMSD bond angle	1.144
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.540	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.165	0.889
Number of reflections	17327	1265
<I/σ(I)>	7.63	1.9
Completeness [%]	99.8	99.4
Redundancy	3.7	3.6
CC(1/2)	0.989	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	298	20 mM Tris-HCl