8ZE8

Arf-GTPase activating protein Asap1 SH3 domain in complex with 440 Kd Ankyrin-B fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 1
Unit cell lengths	39.922, 52.074, 56.909
Unit cell angles	111.42, 90.16, 112.46

Refinement procedure

Resolution	32.400 - 2.070
R-factor	0.1859
Rwork	0.184
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jv3
RMSD bond length	0.013
RMSD bond angle	1.284
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.490	2.120
High resolution limit [Å]	2.070	2.070
Rmerge	0.121	0.213
Rmeas	0.146	0.264
Rpim	0.080	0.153
Total number of observations	75254	4758
Number of reflections	23173	1695
<I/σ(I)>	8.1	5.7
Completeness [%]	97.6
Redundancy	3.2	2.8
CC(1/2)	0.941	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M MES monohydrate, pH=6.0, 20 % w/v Polyethylene glycol 6,000