8ZDX

Crystal structure of MjHKU4r-CoV-1 RBD bound to hDPP4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	74.980, 121.901, 144.664
Unit cell angles	90.00, 93.82, 90.00

Refinement procedure

Resolution	19.690 - 2.600
R-factor	0.2099
Rwork	0.208
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.485
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.750
High resolution limit [Å]	2.600	2.600
Number of reflections	79444	12098
<I/σ(I)>	8.85
Completeness [%]	99.0
Redundancy	6.95
CC(1/2)	0.997	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES (pH 7.0) and 10% (wt/vol) PEG 6000