8ZCL

Ambient Temperature Crystal Structure of Fc Fragment of Human IgG1 from Biosimilar VEGF-Trap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	298
Detector technology	PIXEL
Collection date	2023-09-20
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	50.470, 79.990, 143.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.990 - 2.600
R-factor	0.1827
Rwork	0.179
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	CrysalisPro
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	143.310	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	38860	2273
<I/σ(I)>	4.42
Completeness [%]	96.9
Redundancy	38.5
CC(1/2)	0.850	0.041

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Bis-Tris pH 6.5, 25% w/v Polyethylene glycol 3350