8Z95

Humanized anti-PEG h6.3 Fab in complex with PEG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	245.365, 43.574, 110.148
Unit cell angles	90.00, 111.70, 90.00

Refinement procedure

Resolution	29.810 - 2.260
R-factor	0.21214
Rwork	0.209
R-free	0.26148
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vl9
RMSD bond length	0.008
RMSD bond angle	1.494
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.350
High resolution limit [Å]	2.260	4.880	2.270
Rmerge		0.018	0.333
Rmeas	0.053	0.022	0.391
Rpim		0.012	0.202
Number of reflections	50690	5209	4989
<I/σ(I)>	14.3
Completeness [%]	99.1	97.9	99.2
Redundancy	3.6	3.3	3.7
CC(1/2)		0.999	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% (w/v) PEG-6000, 1% (w/v) PEG-2000-methyl ether, 0.15 M lithium sulfate monohydrate, and 0.1 M citric acid