8Z8L

structure of IL-4Ra in complex with an nanobody 4E9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	99
Detector technology	PIXEL
Collection date	2023-01-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	H 3 2
Unit cell lengths	142.936, 142.936, 149.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.820 - 3.960
R-factor	0.3028
Rwork	0.301
R-free	0.33370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.672
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.200	4.210
High resolution limit [Å]	3.960	3.960
Number of reflections	4534	227
<I/σ(I)>	7.2
Completeness [%]	92.1
Redundancy	12.4
CC(1/2)	0.990	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	1.4 M Sodium malonate dibasic monohydrate pH 6.0