8Z57

Crystal structure of human Q140L-SIRT5 in complex with succinylated Prx1 fragment and ADP ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	46.084, 73.043, 162.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.522 - 1.960
R-factor	0.2013
Rwork	0.199
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.610	2.010
High resolution limit [Å]	1.960	1.960
Rmeas	0.082	1.051
Number of reflections	20158	1361
<I/σ(I)>	15.5	2.2
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.999	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% PEG 3350, 0.1M Tris-HCl pH 8.5