8Z50
Crystal structure of the ASF1-H3T-H4 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-09-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.940, 103.873, 85.315 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.960 - 2.800 |
R-factor | 0.2092 |
Rwork | 0.206 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.117 | 0.057 | 0.599 |
Rmeas | 0.125 | 0.061 | 0.642 |
Rpim | 0.043 | 0.022 | 0.228 |
Total number of observations | 92323 | ||
Number of reflections | 11137 | 620 | 553 |
<I/σ(I)> | 5.6 | ||
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 8.3 | 7.5 | 7.6 |
CC(1/2) | 0.999 | 0.996 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.0 M Lithium chloride, 0.1 M MES pH 6.0, 10% PEG 6000 |