8Z22

Crystal structure of the liprin-alpha2/RIM1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	58.663, 92.697, 66.583
Unit cell angles	90.00, 102.95, 90.00

Refinement procedure

Resolution	38.800 - 2.750
R-factor	0.2065
Rwork	0.205
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.149	0.050	1.151
Rmeas	0.163	0.054	1.264
Rpim	0.065	0.022	0.513
Total number of observations	112901
Number of reflections	18054	932	914
<I/σ(I)>	4.3
Completeness [%]	98.9	98.2	98
Redundancy	6.3	6	5.9
CC(1/2)	0.995	0.998	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	28% V/V 2-Propanol; 0.1M BIS-TRIS pH 6.5; 3% V/V Polyethylene glycol 200