Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.097, 158.982, 179.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.540 - 2.740 |
| R-factor | 0.1754 |
| Rwork | 0.173 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.675 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | FRFS |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.540 | 2.838 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.153 | |
| Rmeas | 0.161 | |
| Rpim | 0.048 | |
| Number of reflections | 75501 | 7561 |
| <I/σ(I)> | 11.57 | |
| Completeness [%] | 95.9 | |
| Redundancy | 10.2 | |
| CC(1/2) | 0.651 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | PEG,magnesium choride,calcium chloride,Ethylene glycol,MES |
