8YR7

Crystal structure of human dishevelled 2 (Dvl2) PDZ domain fused with WGEF internal peptide motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2024-02-04
Detector	RAYONIX MX-225
Wavelength(s)	0.978930
Spacegroup name	I 41
Unit cell lengths	59.491, 59.491, 58.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 3.000
R-factor	0.3011
Rwork	0.299
R-free	0.33500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.361
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.070	3.190
High resolution limit [Å]	3.000	3.000
Rmerge	0.058	1.091
Number of reflections	2071	336
<I/σ(I)>	27.8	2.7
Completeness [%]	99.9	100
Redundancy	14.9	15.1
CC(1/2)	1.000	0.731

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1M Sodium acetate (pH 4.5), 3M Sodium chloride