8YPE
The crystal structure of inactive p38 complexed with a ATF2 from 46 to 80
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.254, 82.254, 123.149 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.570 - 1.950 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_4958) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 1.590 |
| Number of reflections | 35556 | 3480 |
| <I/σ(I)> | 48.75 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.7 | 9.3 |
| CC(1/2) | 1.000 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20% PEG 3350, 0.2M sodium citrate |
