8YP5

The structure of MAP2K4 complexed with 5Z7-oxozeaenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	68.810, 118.032, 81.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.800 - 2.500
R-factor	0.2371
Rwork	0.233
R-free	0.32580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.297
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.032	2.650
High resolution limit [Å]	2.500	2.500
Number of reflections	11757	1849
<I/σ(I)>	10.25
Completeness [%]	99.5
Redundancy	7
CC(1/2)	0.991	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	0.1 M imidazol-HCl pH6.5, 25% PEG 3350