8YMK

Localized reconstruction of Hepatitis B virus surface antigen dimer in the subviral particle with D2 symmetry from dataset A0

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.7
Number of Particle	10324877
Software	RELION,cryoSPARC,PHENIX,SerialEM,CTFFIND,UCSF Chimera
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION