8YLE

Crystal structure of Werner syndrome helicase complexed with AMP-PCP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	49.663, 89.561, 54.359
Unit cell angles	90.00, 102.88, 90.00

Refinement procedure

Resolution	30.050 - 1.860
R-factor	0.1849
Rwork	0.183
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.050	1.926
High resolution limit [Å]	1.860	1.860
Rmerge	0.139	1.123
Rmeas	0.159
Rpim	0.075
Number of reflections	38310	3782
<I/σ(I)>	8.28	2.23
Completeness [%]	98.3	97.54
Redundancy	4.3	4.4
CC(1/2)	0.993	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	pH 6.5,0.1 M Amino acids,0.1 M Buffer System 1, 30.00% % v/v EDO_P8K