Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99977 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 214.362, 50.260, 64.906 |
Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
Resolution | 102.500 - 2.790 |
R-factor | 0.2421 |
Rwork | 0.240 |
R-free | 0.28790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.366 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.500 | 3.040 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.046 | 0.446 |
Number of reflections | 16388 | 3721 |
<I/σ(I)> | 19.3 | |
Completeness [%] | 97.5 | 98.9 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% w/v PEG 3350, 0.2M (NH4)2 TARTRATE |