8YGW
The Crystal Structure of MAPK11 from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.95374 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.061, 61.860, 147.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.150 - 3.301 |
| Rwork | 0.205 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.685 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 3.570 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.199 | 0.827 |
| Number of reflections | 5815 | 1159 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Bis-Tris pH6.5, 28% PEGMME2,000 |
