8YDD

Crystal structure of Nanog(R100A) in complex with Wdr5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	85.667, 87.604, 96.874
Unit cell angles	90.00, 91.81, 90.00

Refinement procedure

Resolution	37.570 - 2.500
R-factor	0.1755
Rwork	0.174
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge		0.074	0.650
Rmeas		0.081	0.703
Rpim		0.031	0.266
Number of reflections	49782	2571	2463
<I/σ(I)>	2.8
Completeness [%]	99.9	99.5	99.9
Redundancy	6.8	6.6	6.9
CC(1/2)	0.803	0.996	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate