8YB5
SARS-CoV-2 DMV nsp3-4 pore complex (consensus-pore, C6 symmetry)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 4.2 |
| Software | emClarity,Coot,ISOLDE,RELION,PHENIX,SerialEM,UCSF Chimera,UCSF ChimeraX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
