8Y31

The crystal structure of the QX006N-Fab/IFNAR1-SD123 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	73.155, 121.031, 79.738
Unit cell angles	90.00, 111.51, 90.00

Refinement procedure

Resolution	46.890 - 2.680
R-factor	0.1965
Rwork	0.194
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.775
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.060	2.830
High resolution limit [Å]	2.680	2.680
Number of reflections	35675	3527
<I/σ(I)>	12.8
Completeness [%]	98.6
Redundancy	5.6
CC(1/2)	0.993	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		289	8%(w/v) PEG3350 50 mM Na-Citrate pH 5.5, 100 mM (NH4)2Citrate, 250 mM NaCl, 5 mM Hexadecyltrimethylammonium Bromide