8XV6
Crystal structure of PHD domain of UHRF1 in complex with mStella peptide (residues 85-119)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.940, 75.720, 96.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.600 |
| R-factor | 0.15307 |
| Rwork | 0.152 |
| R-free | 0.16803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.810 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.038 | 0.321 |
| Rmeas | 0.042 | 0.361 |
| Rpim | 0.017 | 0.162 |
| Total number of observations | 213799 | 8346 |
| Number of reflections | 34602 | 1748 |
| <I/σ(I)> | 21 | 3.9 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.2 | 4.8 |
| CC(1/2) | 0.999 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M Potassium thiocyanate, 30% PEG MME2000 |
