8XPT
The Crystal Structure of EHMT1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95355 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.113, 94.638, 88.731 |
| Unit cell angles | 90.00, 112.34, 90.00 |
Refinement procedure
| Resolution | 48.415 - 3.350 |
| Rwork | 0.195 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.086 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.415 | 3.620 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.256 | 0.832 |
| Number of reflections | 16513 | 3495 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 88.0 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Li2SO4, 0.1M Tris-HCl pH8.5, 25% PEG MME 5000 |
