8XOM
Cryo-EM structure of human ABCC4 in complex with ANP-bound in NBD1 and METHOTREXATE
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.05 |
| Number of Particle | 5036677 |
| Software | SerialEM |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
