8XND

Crystal structure of serine hydroxymethyltransferase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.18057
Spacegroup name	P 32
Unit cell lengths	151.540, 151.540, 234.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.530 - 3.450
R-factor	0.15499
Rwork	0.153
R-free	0.19205
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.693
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.530	3.520
High resolution limit [Å]	3.450	3.450
Rmerge	0.161
Number of reflections	79382	4531
<I/σ(I)>	10.4
Completeness [%]	99.9
Redundancy	5.3
CC(1/2)	0.995	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium HEPES pH 7.5,30 %(v/v) PEG 300