8XJ0

Crystal structure of AmFab mutant - P40C/E165C (Light chain), G10C/P210C(Heavy chain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2023-06-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	2.7000
Spacegroup name	P 21 21 21
Unit cell lengths	47.617, 193.079, 220.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 3.300
R-factor	0.19
Rwork	0.186
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.192
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.460	3.480
High resolution limit [Å]	3.300	3.300
Rpim	0.074	0.336
Number of reflections	31855	4547
<I/σ(I)>	13.2	3
Completeness [%]	99.9
Redundancy	37.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	18% PEG 3350, 0.1 M Tris-HCl pH 7.5-8.0, 0.2 M calcium chloride