8XC4

Nipah virus attachment glycoprotein head domain in complex with a broadly neutralizing antibody 1E5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	193.422, 193.422, 198.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.690 - 3.240
R-factor	0.219
Rwork	0.218
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vy5
RMSD bond length	0.005
RMSD bond angle	1.109
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.320
High resolution limit [Å]	3.240	3.240
Rmerge	0.189	0.189
Number of reflections	58116	58116
<I/σ(I)>	9.57
Completeness [%]	97.1
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1.5 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH4.6