8X6B

Crystal structure of immune receptor PVRIG in complex with ligand Nectin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 43 21 2
Unit cell lengths	80.974, 80.974, 82.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.490 - 2.000
R-factor	0.2058
Rwork	0.204
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19rc3_4028)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.870	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.822
Number of reflections	26249	3764
<I/σ(I)>	23.5	3.2
Completeness [%]	100.0
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.6 M magnesium sulfate heptahydrate, 0.1 M MES