8X5K
The Crystal Structure of SYK from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.095, 83.903, 39.962 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 41.951 - 1.800 |
| Rwork | 0.189 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.274 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.951 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 | 0.763 |
| Number of reflections | 22844 | 1374 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Li2SO4, 0.1M Bis-Tris pH6.5, 25% PEG 3350 |
