8WWR
Crystal structure of apo human dishevelled 2 (Dvl2) PDZ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-27 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.978, 45.978, 78.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.510 - 1.750 |
| R-factor | 0.2282 |
| Rwork | 0.227 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.510 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.731 |
| Number of reflections | 9023 | 482 |
| <I/σ(I)> | 23.3 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.5 | 15.9 |
| CC(1/2) | 0.999 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tri-sodium citrate, pH 5.6 20% (v/v) Isopropanol 20%(w/v) PEG4000 |
