8WTF
The Crystal Structure of IRAK4 from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.449, 93.061, 60.925 |
| Unit cell angles | 90.00, 99.15, 90.00 |
Refinement procedure
| Resolution | 46.530 - 2.000 |
| Rwork | 0.180 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.151 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.147 | 0.515 |
| Number of reflections | 19106 | 1425 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate,0.1M BisTris,5.5,25% w/vPEG 3350 |
