8WSQ
A protective human antibody against respiratory syncytial virus by targeting a prefusion epitope across sites IV and V of the viral fusion glycoprotein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 183.656, 183.656, 183.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.080 - 2.900 |
| R-factor | 0.2488 |
| Rwork | 0.247 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.716 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.080 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 22923 | 2243 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 38.7 | |
| CC(1/2) | 0.900 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 291 | 0.15 M DL-Malic acid pH 6.2, 0.1 M Imidazole pH 7.0, 27% (v/v) polyethylene glycol monomethyl ether 550 |
