8WHL
Crystal structure of CLASP2 in complex with CENP-E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 |
Unit cell lengths | 106.221, 106.221, 327.051 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.890 - 3.200 |
R-factor | 0.2479 |
Rwork | 0.246 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.117 | 0.070 | 1.035 |
Rmeas | 0.128 | 0.077 | 1.130 |
Rpim | 0.051 | 0.031 | 0.449 |
Total number of observations | 217234 | ||
Number of reflections | 34412 | 1762 | 1705 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.9 | 99.5 | 99.9 |
Redundancy | 6.3 | 6.1 | 6.3 |
CC(1/2) | 0.991 | 0.993 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M HEPES pH 6.5 and 1.4 M sodium citrate |