8WHI

Crystal structure of native CLASP2 in complex with CLIP170

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	52.624, 85.545, 114.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.312 - 1.853
R-factor	0.1933
Rwork	0.191
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.107	0.060	1.686
Rmeas	0.116	0.065	1.835
Rpim	0.044	0.025	0.714
Total number of observations	312929
Number of reflections	44523	2422	2189
<I/σ(I)>	6.4
Completeness [%]	100.0	99.8	99.9
Redundancy	7	6.5	6.8
CC(1/2)	0.997	0.997	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M NaCl, 0.1 M Tris pH 8.5, and 25% w/v PEG 3,350