8WEF

Crystal structure of Brassica napus MIK2 ectodomain in complex with Fusarium oxysporum SCOOPL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-04-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	155.959, 155.959, 125.087
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.710 - 2.800
R-factor	0.1862
Rwork	0.185
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.658
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	43756	4172
<I/σ(I)>	12.2
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 34% w/v PEG 400