8W78
Structure of Drosophila melanogaster L-2-hydroxyglutarate dehydrogenase in complex with FAD and 2-oxoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.082, 102.330, 102.632 |
Unit cell angles | 90.00, 108.33, 90.00 |
Refinement procedure
Resolution | 31.670 - 2.810 |
R-factor | 0.2305 |
Rwork | 0.228 |
R-free | 0.27730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.471 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.870 |
High resolution limit [Å] | 2.810 | 7.650 | 2.820 |
Rmerge | 0.174 | 0.034 | 1.430 |
Rmeas | 0.189 | 0.037 | 1.566 |
Rpim | 0.072 | 0.014 | 0.626 |
Number of reflections | 47660 | 2470 | 2353 |
<I/σ(I)> | 3.8 | ||
Completeness [%] | 99.4 | 99.2 | 98.6 |
Redundancy | 6.7 | 6.6 | 5.9 |
CC(1/2) | 0.994 | 0.999 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2 M magnesium formate and 18% (w/v) PEG 3350, 40 mM 2-OG |