8W1V
The beta2 adrenergic receptor bound to a bitopic ligand
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-07-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.680, 146.770, 91.830 |
| Unit cell angles | 90.00, 107.06, 90.00 |
Refinement procedure
| Resolution | 48.440 - 3.000 |
| R-factor | 0.2501 |
| Rwork | 0.248 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.705 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.440 | 3.100 |
| High resolution limit [Å] | 2.920 | 3.000 |
| Number of reflections | 40547 | 6655 |
| <I/σ(I)> | 8.25 | |
| Completeness [%] | 99.9 | |
| Redundancy | 55.5 | |
| CC(1/2) | 0.984 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM Tris buffer (pH 8.0), 100 to 175 mM lithium sulfate, 38% to 42% PEG400, and 10 mM EDTA |
