8W0Z
Human LCAD complexed with Lauric Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.288, 94.842, 119.163 |
| Unit cell angles | 89.24, 74.88, 88.35 |
Refinement procedure
| Resolution | 30.120 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.080 | 0.049 | 0.506 |
| Total number of observations | 604786 | ||
| Number of reflections | 243769 | 11600 | 11015 |
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 96.1 | 91.3 | 86.8 |
| Redundancy | 2.5 | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 291 | TrisHCl, PEG5000MME, NaCl, MgCl2, beta-octyl-b-D-glucoside |
