8W0Y
Crystal structure of broadly neutralizing antibody hcab17 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2021-09-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 179.410, 179.410, 168.762 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.690 - 3.310 |
R-factor | 0.2115 |
Rwork | 0.209 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.098 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.710 | 3.430 |
High resolution limit [Å] | 3.310 | 3.310 |
Rmerge | 0.483 | 3.032 |
Rmeas | 0.510 | 3.216 |
Rpim | 0.163 | 1.063 |
Total number of observations | 459905 | 40627 |
Number of reflections | 47162 | 4577 |
<I/σ(I)> | 3.7 | 1.1 |
Completeness [%] | 100.0 | |
Redundancy | 9.8 | 8.9 |
CC(1/2) | 0.970 | 0.235 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.0 M Sodium citrate tribasic dihydrate and 0.1 M Sodium cacodylate |