8VZZ
Q108K:K40L:T51V:T53S:Y19W:R58W mutant of hCRBPII bound to synthetic fluorophore TD-1V
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-24 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 29.329, 36.432, 64.083 |
Unit cell angles | 89.74, 89.36, 66.38 |
Refinement procedure
Resolution | 29.600 - 1.220 |
Rwork | 0.179 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qyn |
RMSD bond length | 0.011 |
RMSD bond angle | 1.447 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 1.290 |
High resolution limit [Å] | 1.220 | 1.220 |
Number of reflections | 72294 | 6768 |
<I/σ(I)> | 44.63 | |
Completeness [%] | 93.5 | |
Redundancy | 13.5 | |
CC(1/2) | 0.889 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG4000, ammonium acetate, sodium acetate |