8VZC
Crystal Structure of 2-Hydroxyacyl-CoA Lyase/Synthse ApbHACS from Alphaproteobacteria bacterium in the Complex with THDP, Formyl-CoA, and ADP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0328 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.084, 102.442, 103.977 |
| Unit cell angles | 113.83, 96.81, 105.45 |
Refinement procedure
| Resolution | 38.630 - 1.720 |
| R-factor | 0.1634 |
| Rwork | 0.162 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.920 | 1.810 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.070 | 0.429 |
| Rmeas | 0.088 | 0.530 |
| Rpim | 0.054 | 0.319 |
| Number of reflections | 238150 | 26539 |
| <I/σ(I)> | 7.2 | 1.8 |
| Completeness [%] | 92.3 | 69.7 |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.996 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M sodium sulfate, 0.1 M Bis-Tris Propane pH 7.5, 20 % (w/v) PEG 3350 |
