8VYD

A novel synthase generates m4(2)C to stabilize the archaeal ribosome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	77
Detector technology	CMOS
Collection date	2021-11-22
Detector	RDI CMOS_8M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	37.670, 97.280, 89.020
Unit cell angles	90.00, 99.64, 90.00

Refinement procedure

Resolution	42.540 - 1.950
R-factor	0.2404
Rwork	0.238
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.126
Data reduction software	HKL-3000
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.540	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.189
Rmeas	0.200
Rpim	0.062
Number of reflections	44882	56908
<I/σ(I)>	14.28
Completeness [%]	84.5	40.2
Redundancy	12.4	12.5
CC(1/2)	0.942	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	PEG 3350, Tris-HCL, NaCl, DTT