8VXJ
The crystal structure of human apolipoprotein A-I in complex with Fab 55201
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.530, 67.472, 167.123 |
| Unit cell angles | 90.00, 95.74, 90.00 |
Refinement procedure
| Resolution | 45.600 - 2.700 |
| R-factor | 0.2679 |
| Rwork | 0.266 |
| R-free | 0.29810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.469 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.145 | 2.358 |
| Rmeas | 0.171 | 2.764 |
| Rpim | 0.090 | 1.432 |
| Number of reflections | 35513 | 4641 |
| <I/σ(I)> | 6.7 | 0.8 |
| Completeness [%] | 98.9 | 98.5 |
| Redundancy | 6.9 | 7.2 |
| CC(1/2) | 0.998 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 15% PEG 6000, 0.1 M trisodium citrate-citric acid, pH 5.5, 0.02% NaN3 |
