8VWX

Human Bcl-2 (G101V Mutant)/Bcl-xL Chimera Fused to Maltose-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	37.343, 89.031, 142.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.480 - 1.770
R-factor	0.1929
Rwork	0.191
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.480	1.833
High resolution limit [Å]	1.770	1.770
Rmerge	0.129
Rmeas	0.140
Rpim	0.052	0.860
Number of reflections	47261	4625
<I/σ(I)>	10.26	0.92
Completeness [%]	100.0	99.94
Redundancy	7.2	7.1
CC(1/2)	0.998	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	100 mM Tris (pH 8.5), 25% PEG 3350, and 200 mM sodium chloride