8VW5

Crystal structure of Cbl-b TKB bound to compound 2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	55.198, 56.820, 71.568
Unit cell angles	102.90, 96.55, 111.97

Refinement procedure

Resolution	35.880 - 1.760
R-factor	0.1767
Rwork	0.175
R-free	0.20410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.638
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.880	1.830
High resolution limit [Å]	1.760	1.760
Number of reflections	72083	6009
<I/σ(I)>	9.35	0.98
Completeness [%]	84.3
Redundancy	2
CC(1/2)	0.998	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ca(OAc)2, 0.1 M NaCacodylate pH 6.5, 18% PEG8000