8VVO
Structure of FabS1CE2-EPR1-1 in complex with the erythropoietin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.246, 90.576, 214.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.460 - 3.090 |
| R-factor | 0.2547 |
| Rwork | 0.253 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.857 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.430 | 3.310 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.358 | 1.807 |
| Rmeas | 0.390 | 1.953 |
| Rpim | 0.152 | 0.734 |
| Number of reflections | 25960 | 4627 |
| <I/σ(I)> | 5.3 | 1 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 6.5 | 6.9 |
| CC(1/2) | 0.987 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1 M MMT buffer pH 6.0, 25% PEG1500 |
