8VVO

Structure of FabS1CE2-EPR1-1 in complex with the erythropoietin receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.246, 90.576, 214.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.460 - 3.090
R-factor	0.2547
Rwork	0.253
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.857
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.430	3.310
High resolution limit [Å]	3.090	3.090
Rmerge	0.358	1.807
Rmeas	0.390	1.953
Rpim	0.152	0.734
Number of reflections	25960	4627
<I/σ(I)>	5.3	1
Completeness [%]	99.2	99.7
Redundancy	6.5	6.9
CC(1/2)	0.987	0.487

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1 M MMT buffer pH 6.0, 25% PEG1500