8VTW

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-128 and protein Y at 2.35A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.600, 449.320, 619.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	124.580 - 2.350
R-factor	0.2087
Rwork	0.207
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhx
RMSD bond length	0.005
RMSD bond angle	0.901
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	124.580	124.580	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.186	0.064	2.577
Rmeas	0.200	0.069	2.749
Total number of observations	18790343
Number of reflections	2389130	27799	175929
<I/σ(I)>	8.67	28.41	0.88
Completeness [%]	100.0	99.1	99.9
Redundancy	7.865	7.847	8.011
CC(1/2)	0.993	0.992	0.182

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME